Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks
نویسندگان
چکیده
منابع مشابه
Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks.
Although recent developments in MS have enabled the identification and quantification of hundreds of phosphorylation sites from a given biological sample, phosphoproteome analysis by MS has been plagued by inconsistent reproducibility arising from automated selection of precursor ions for fragmentation, identification, and quantification. To address this challenge, we have developed a new MS-ba...
متن کاملQuantitative proteomic analysis of phosphotyrosine-mediated cellular signaling networks.
Receptor tyrosine kinases receive extracellular cues, such as ligand binding, and transmit this information to the cell through both autophosphorylation and phosphorylation of tyrosine residues on selected substrates, stimulating a variety of signal transduction pathways. Quantitative features, including intensity, timing, and duration of phosphorylation of particular residues, may play a role ...
متن کاملQuantitative Analysis of Signaling Networks
Glioblastoma (GBM) is the most common malignant form of brain cancer. Even with treatment including surgery, radiation, and temozolomide chemotherapy, the 1 year survival rate is only 35%. To identify specific mediators of GBM progression in a genetically engineered murine model of proneural GBM, we quantified signaling networks using mass spectrometry. We identified oncogenic signaling associa...
متن کاملQuantitative analysis of signaling networks.
The response of biological cells to environmental change is coordinated by protein-based signaling networks. These networks are to be found in both prokaryotes and eukaryotes. In eukaryotes, the signaling networks can be highly complex, some networks comprising of 60 or more proteins. The fundamental motif that has been found in all signaling networks is the protein phosphorylation/dephosphoryl...
متن کاملThe Database of Quantitative Cellular Signaling: management and analysis of chemical kinetic models of signaling networks
MOTIVATION Analysis of cellular signaling interactions is expected to pose an enormous informatics challenge, perhaps even larger than analyzing the genome. The complex networks arising from signaling processes are traditionally represented as block diagrams. A key step in the evolution toward a more quantitative understanding of signaling is to explicitly specify the kinetics of all chemical r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2007
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0608638104